As anaerobes such as Porphyromonas gingivalis are difficult to culture, VPC becomes time‐consuming and less sensitive. The viable plate count (VPC) is considered as the reference method for bacterial enumeration in periodontal microbiology but shows some important limitations for anaerobic bacteria.In this study methylene blue reduction test and standard plate count plate count Subject Category: Properties The small amount of equipment and materials and the simplicity of the method are the most important advantages of this test.
